Abstract

VELOZA, L. C. et al. PRESENCE OF LMP-1 PROTEIN OF EPSTEIN-BARR VIRUS IN LYMPHOMAS OF CANINE ORIGIN. Rev. Med. Vet. Zoot. [online]. 2017, vol.64, n.3, pp.11-21. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v64n3.68691.

Epstein Barr virus (EBV) is a human high prevalent virus associated with lymphoid B cells tumors development. In canines, few reports have been published regarding the presence of the virus in dogs but its role in this species remains unclear. The aim of this study was to determine the presence of LMP-1 protein of EBV in 20 canine lymphomas tissues which were previously diagnosed in a period of time between 2004 -2014. Lymphomas were reclassified in accordance with the new histopathological classifications for lymphomas and were stained by IHQ with anti-CD79a, anti-CD3 and anti-LMP-1; in addition, specific antibodies for B lymphocytes, T lymphocytes and EBV biomarker, respectively. It was found that the most common lymphoma was T-zone lymphoma in 75% of the cases of the study. The distribution of the cases regarding the immunostaining was: 18 positive cases with anti-CD3, 2 positive cases with anti-CD79a and 6 positive cases with anti- LMP-1. Positive cases of LMP-1 as a biomarker of the presence of EBV corresponded to the 30% of the cases of the study. Chi-square test showed statistical significance between the presence of the virus and the presence of lymphomas, which suggests not only that the virus is circulating in the canine population but also that could have implications in the development of the disease. Regarding demographic parameters, only the Golden Retriever breed showed a relationship with the presence of lymphoma, but not with the presence of the virus.

Keywords : Epstein-Barr virus (EBV); lymphoma; canines; LMP-1; CD3; CD79a.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )