Abstract

JURADO-GAMEZ, H.; SINSAJOA-TEPUD, M.  and  NARVAEZ-RODRIGUEZ, M.. Evaluation of microencapsulated Lactobacillus plantarum and its viability under simulated gastrointestinal conditions and inhibition against Escherichia coli O157: H7. Rev. Med. Vet. Zoot. [online]. 2019, vol.66, n.3, pp.231-244. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v66n3.84260.

The viability of microencapsulated Lactobacillus plantarum, its inhibition effect on Escherichia coli O157: H7 and growth in simulated gastrointestinal conditions was evaluated. L. plantarum ATCC 8014® and E. coli ATCC 43888® were used. Inhibition tests of L. plantarum on E. coli and susceptibility test to CTX (30 µg), P (10 IU), GN (10 µg) DCX (1 µg), CIP (5 µg) and KF (30) were performed µg) for both strains. In the lactic bacterium the fermentation kinetics were determined, and the presence of peptides and amino acids by HPLC in both strains. The growth at 37 ° C and 45 ° C of L. plantarum was evaluated and the viability of its microencapsulation was assessed by gastrointestinal conditions (Bile, Bile salts and acidic pH), as well as the survival and stability of the preparation and its physical characteristics and morphological. The results indicated that L. plantarum inhibited E. coli and the microencapsulated positive results with a viability of 83.3%, efficiency 88.4%, Humidity 7.79%, water activity 0.4, wettability of 1 min, 56 s, 96% solubility, spherical morphology and size between 15.18 to 35.68 pm. Finally, a high potential of L. plantarum was observed as an inhibitory agent for E. coli O157: H7.

Keywords : L. plantarum; E. coli O157: H7; food safety; public and animal health.

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