SciELO - Scientific Electronic Library Online

vol.34 suppl.1Mobile genetic elements associated with erythromycin-resistant isolates of Streptococcus pneumoniae in ColombiaThe spread of KPC-3 Klebsiella pneumoniae in hospitals in Bogotá over a three-year period (2008-2010) índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados



Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google



versão impressa ISSN 0120-4157


SAAVEDRA, Sandra Yamile; DUARTE, Carolina; GONZALEZ, María Nilse  e  REALPE, María Elena. Characterization of isolates of carbapenemase-producing Pseudomonas aeruginosa from seven Colombian provinces . Biomédica [online]. 2014, vol.34, suppl.1, pp.217-223. ISSN 0120-4157.

Introduction: Pse udomonas aeruginosa is an opportunistic pathogen that causes multiple infections in hospitalized patients. This microorganism has developed resistance to several antimicrobial agents, including carbapenems, which are considered to be the last therapeutic option against these infections. Carbapenem resistance of P. aeruginosa is mediated by different mechanisms: Carbapenemases class B (MBL) and A, alterations in the OprD expression and overexpression of the Mex efflux pump. Objective: To describe the presence of carbapenemases in P. aeruginosa isolates from seven Colombian provinces. Materials and methods: A total of 57 P. aeruginosa isolates were collected between September 2012 and March 2013 from national surveillance in Colombia and were sent to the Grupo de Microbiología at the Instituto Nacional de Salud (INS) for evaluation. Iidentification and antimicrobial susceptibility were confirmed through automated method (Vitek ® 2) and disk diffusion (Kirby-Bauer) according to the Clinical and Laboratory Standards Institute, CLSI, 2013. Phenotypic and genotypic confirmation was determined using the modified Hodge test (MHT), a synergism test using imipenem, EDTA-SMA and meropenem, and conventional PCR to detect the bla KPC, bla VIM, bla IMP and bla NDM genes. Results: Of the 57 isolates, two showed sensitivity to carbapenems. Forty-three isolates were positive for carbapenemases with a high percentage of sensitivity to colistin (76.4%, n=42). The 43 isolates producing carbapenemases showed multiple drug resistance: 72.1% were positive in the MHT and 79.1% showed MBL synergism. PCR amplification confirmed that 33 isolates were positive for bla VIM, nine were positive for bla KPC and one isolate expressed both KPC and VIM carbapenemases. No isolates showed amplified products with bla IMP and bla NDM primers. Conclusions: The most frequent carbapenemase was VIM, followed by KPC in an approximate ratio of 3:1.

Palavras-chave : Pseudomonas aeruginosa; carbapenems; drug resistance, microbial.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )