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Biomédica
Print version ISSN 0120-4157
Abstract
LOPEZ-RUBIO, Andrés et al. Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913. Biomédica [online]. 2017, vol.37, suppl.2, pp.143-154. ISSN 0120-4157. https://doi.org/10.7705/biomedica.v34i2.3452.
Introduction:
Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms.
Objective:
The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai.
Materials and methods:
Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene.
Results:
Thirty-six sequences for A. neivai and A. pholidotus were obtained.
Conclusions:
Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected.
Keywords : Anopheles; malaria; RNA, transfer; DNA, mitochondrial; Colombia; Panamá; Guatemala.
