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Biomédica

versão impressa ISSN 0120-4157versão On-line ISSN 2590-7379

Resumo

LEON-LUNA, Diana et al. Molecular characterization of multiresistant enterobacteria in two departments of the Peruvian jungle. Biomed. [online]. 2021, vol.41, suppl.2, pp.180-187.  Epub 15-Out-2021. ISSN 0120-4157.  https://doi.org/10.7705/biomedica.5720.

Introduction.

The emergence of multiresistant enterobacteria producing extended- spectrum beta-lactamase (ESBL) in outpatients with urinary tract infections represents a public health problem in Perú.

Objectives.

To characterize multiresistant enterobacteria isolated from patients diagnosed with urinary tract infection in two Peruvian jungle departments using molecular techniques.

Materials and methods.

We conducted a descriptive, observational, and retrospective study of 61 urine culture isolates from two departments in the Peruvian jungle during 20172018. Resistance profiles were identified using the MicroScan™ automated system and a conventional polymerase chain reaction (PCR) was used for the detection of bla CTX-M , bla TEM and bla SHV genes..

Results.

The most common positive ESBL enterobacteria for each department were Escherichia coli in Madre de Dios (10/40; 25%) and Ucayali (16/21; 76.2%). Gene bla CTX-M was the most prevalent in both departments (25/61; 41%), followed by bla TEM (15/61; 24.6%), and bla SHV (10/61; 16.4%). As for the antimicrobial susceptibility profile, we detected resistance levels of 72.6% for ampicillin, 82.3% for cephalothin, and 88.7% for nitrofurantoin.

Conclusions.

BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4% and bla CTX-M was the most common gene.

Palavras-chave : Enterobacteriaceae; drug resistance, microbial; beta-lactam resistance; genes.

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