Resumo

RODRIGUEZ, M; BOTERO, E; IREGUI, CA  e  FIGUEROA, J. Aeromonas hydrophila Extracelullar Products Extraction and its Effects on Tilapia Roja (Oreochromis spp.) and Cachama Blanca (Piaractus brachypomus). Acta biol.Colomb. [online]. 2005, vol.10, n.2, pp.75-94. ISSN 0120-548X.

A toxigenic raw extract of A. hydrophila was obtained from diverse culture medium (BHI with extract of yeast, (TSB) y minimum salt medium (MSM)) and diverse concentration techniques. Extracts were processed by Sephadex A-50 (QAE) and SDS-PAGE electrophoresis. Biological activity was measured in vitro (hemolytic and proteolitic activity) and in vivo (toxicity in fish). The results indicate that the optimal culture condition was 28 ºC and MSM was the optimum medium to extract. Dehydration at 4 ºC was the most adequate concentration technique. TBS extract obtained had an hemolytic activity of 128 UH/µL and a proteolitic activity of 38.4 UP/µL and produced high fish mortality and severe multiorganic lesions. Electrophoresis revealed bands from 50 to 52 kDa y 63 to 68 kDa, that could correspond to the β hemolisina or α hemolisina y other from 30 to 36 kDa that could correspond to the thermostable metalloprotease.

Palavras-chave : Aeromonas hydrophila; extracelular products; hemolisins; haemorragic septicemia.

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