Acta Biológica Colombiana
Print version ISSN 0120-548X
RIVERA R., CARLOS et al. Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a. Acta biol.Colomb. [online]. 2005, vol.10, n.2, pp. 95-103. ISSN 0120-548X.
In four mountain aquatic ecosystems of altiplano Cundiboyacense, Colombia, we compared through analysis regression data set of chlorophyll-a obtained with spectorophotometric (tricromatic formula) and fluorometric (Welschmeyer) methods. The analysis showed that the spectrophotometric method overestimated the chlorophyll-a concentration, but it could be used with precaution in the lower trophic environments. An equation that relates the two chlorophyll-a measures was obtained.
Keywords : Chlorophyll-a; spectrophotometry; fluorometry; wetlands; mountain aquatic ecosystems.