Resumo

CASTANEDA, NADIA Y; CASTELLANOS, JAIME E; ZAPATA, ANGELA C  e  BELLO, FELIO. AEGY28 Cell Line of Aedes aegypti (Diptera Culicidae) is Infection Refractory to Dengue 2 and Yellow Fever Virus. Acta biol.Colomb. [online]. 2007, vol.12, n.2, pp.47-58. ISSN 0120-548X.

Mosquito cell derived cultures are useful tools for arbovirus isolation, identification or characterization. For studying dengue (DENV) and yellow fever viruses (YFV) Aedes albopictus C6/36 or Aedes pseudoscutellaris AP61 cell lines, are normally used. The Aedes aegypti AEGY28 cell line was obtained from embryonic tissues and characterized previously by one of us. In order to evaluate its susceptibility to two Flavivirus, AEGY28 cells were inoculated with different multiplicity of infection (MOI) with type 2 DENV (COL789, MOI: 1 and 5) and YFV clinical isolates (V341, MOI 0,02) then processed at different times post infection (p.i.). Immunostaining and fluorometric cellELISA were carried out to identify and quantify viral antigens. C6/36 and Vero cells were used as positive controls. Unexpectedly, immunoreactivity was not found in inoculated AEGY28 cells, even in higher MOI or late times p.i., therefore antigen quantification using fluorometric cellELISA were not plausible. Reverse transcriptase PCR with specific primers did not detect viral RNA in AEGY28 inoculated cells. We can conclude that Aedes aegypti AEGY28 cell line is not susceptible to dengue and yellow fever Flavivirus, a finding possibly related with the lacking of specific molecules at the plasma membrane or absence of cell machinery necessary for viral replication

Palavras-chave : Flavivirus; cell line; dengue virus; yellow fever; arboviruses; infection.

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