Abstract

YAYA-LANCHEROS, MARY LUZ  and  CHAPARRO- GIRALDO, ALEJANDRO. REGENERATION AND MANNOSE SENSIBILITY IN POTATO INTERNODAL EXPLANTS (Solanum tuberosum spp. andígena Var Diacol Capiro). Acta biol.Colomb. [online]. 2009, vol.14, n.1, pp.143-154. ISSN 0120-548X.

A system of indirect organogenesis for the multiple buds production from internode stem sections in Diacol Capiro variety was established. Explants on Murashige & Skoog (MS) medium with zeatine riboside (ZR) 2 mg/l, naftalenacetic acid (NAA) 0.02 mg/l and giberelic acid (GA3) 0.02 mg/l, produced plants ranging between 7 to 9 weeks with 80-100% effectiveness. In the same medium, explants infected with Agrobacterium LBA4404 strain which carries recombinant plasmid pNOV022, produced regeneration rates reached 100%, thus, the medium utility for trnsformation processes was verified. Finally, to determine the potential use of the mannose as selective agent in transformation processes, the effect of different mannose concentrations on explant viability and regenerative capacity was evaluated.

Keywords : Indirect organogenesis; positive selection; transgenic plants; Agrobacterium tumefaciens.

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