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Acta Biológica Colombiana

Print version ISSN 0120-548X

Abstract

MENDEZ, JONH J; RAMON, CANELA  and  TORRES, MERCÈ. KINETIC STUDY OF PALMITIC ACID ESTERIFICATION CATALYZED BY Rhizopus oryzae RESTING CELLS. Acta biol.Colomb. [online]. 2009, vol.14, n.1, pp.161-172. ISSN 0120-548X.

In the present study, a kinetic model for the biocatalytic synthesis of esters using Rhizopus oryzae resting cells is proposed. The kinetic study has been made in a range of 30-50 °C and atmospheric pressure. The Influence of operating variables, water content, pH, amount of mycelium was studied. Different values of temperature, initial mycelium concentration and acid/alcohol molar ratio were tested. Initial rates were estimated from the slope of the concentration of palmitic acid, or their corresponding ester at conversions of less than 10%, versus time and reported as mmol l-1 min -1. The values of kinetic constants were computed using the freeware program SIMFIT (http:\\www.simfit.man.ac.uk).

Keywords : bound lipase; esterification; fungal resting cells; Rhizopus oryzae; palmitic acid; propanol.

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