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Acta Biológica Colombiana

Print version ISSN 0120-548X

Abstract

GAMBOA ABRIL, M. VIVIANA  and  FIGUEROA RAMIREZ, JUDITH. Antibacterial Power Of Honeys From Tetragonisca angustula Assessed By Minimum Inhibitory Concentration. Acta biol.Colomb. [online]. 2009, vol.14, n.2, pp.97-106. ISSN 0120-548X.

The in vitro antibacterial capacity was determined for 37 honeys of Tetragonisca angustula from seven regions of Colombia, by minimum inhibitory concentration (CMI) against three positive Gram bacteria and three negative Gram bacteria. The CMI technique was evaluated by microdilutions (v/v) to 90%, 45%, 22.5%, 12.3%, 5.6% of honey against the negative Gram bacteria, Salmonella enterica sp. enteric serovar Typhimurium, ATCC 1402; Escherichia coli, 31617 ATCC and Klebsiella pneumoniae subsp. pneumoniae, ATCC 700603 and to positive Gram bacteria, Bacillus subtilis sp. spizizenii, ATCC 6633; Staphylococcus aureus sp. aureus Rosenbach, 6538 ATCC and Micrococcus luteus Kocuria rhizophila, ATCC 9341. The honey samples corresponded to Colombia regions: Antioquia, Cauca, Cundinamarca, Magdalena, Santander, Tolima and Valle del Cauca. The honeys analyzed by binomial test showed bactericidal effect against the tested bacteria; the greater effectiveness was detected in E. coli, M. luteus and S. enterica, with probability or p(x)=1 in a 90% dilution; for followed up dilutions, the effect was still demonstrated against M. luteus in a 5.6% dilution with an inhibition activity from honeys at p(x)=0.62. In a 90% dilution, the effect was only closed to p(x)=0.5, for K. pneumoniae, B. subtilis and S. aureus. The region of Antioquia, exhibits honeys with superior bactericidal action between the sampling areas.

Keywords : honey; Tetragonisca angustula; CMI.

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