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Acta Biológica Colombiana

Print version ISSN 0120-548X

Abstract

REY, MAURICIO; USAQUEN, WILLIAM; ACOSTA, EDWIN  and  PARRA, JULIO. Population Data Of Human DNA Microsatellites D2S1338, D19S433, Penta D, Penta E And SE-33 From Central Region In Colombia. Acta biol.Colomb. [online]. 2009, vol.14, n.2, pp.161-168. ISSN 0120-548X.

Microsatellites (STR's) are short tandem repeat sequences of nucleotides through DNA, very important for their polymorphic character that allow their utilization in population genetic identification. The STR's markers D2S1338, D19S433, PENTA D, PENTA E y SE33 were used to do the genetic profiles of human populations from the Cundiboyacense region in Colombia. The five STR's were amplified by PCR (polymerase chain reaction), indentified with capillary electrophoresis, and typified with Genscan and Genotyper software. Allelic frequencies of the five microsatellites are reported (not previously reported for this region). It was found that loci D19S433 y SE-33 are not in Hardy-Weinberg equilibrium; and Penta E provides the greatest power of discrimination. The allelic frequencies distribution to these five markers, in the two populations studied show that it does not exist statistically significant differences between them.

Keywords : STR; D2S1338; D19S433; PENTA D; PENTA E; SE-33.

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