Abstract

PUCCI, GRACIELA et al. Diversity Of Cultivable Bacteria From The Coast Of Caleta Olivia, Patagonia , Argentina. Acta biol.Colomb. [online]. 2009, vol.14, n.3, pp.121-134. ISSN 0120-548X.

The aim of this work was to evaluate the oil utilization and identity of the bacterial strains present in the coast Intertidal sediments and marine water from Caleta Olivia city which were analyzed to determine bacterial counts. Five microcosms were designed for sediments and water sample. The microcosms contained 5 g or 50 mL of samples with 0.01% of gasoline, and 0.1% of kerosene, diesel, crude oil and mineral oils. The CO2 was measured by titration. Four cultural medium were used i.e. BBR, BRN, mineral medium with crude oil and gas oil and ENDO for coliforms. The bacteria were identified by Sherlock - MIDI . The mineralization shows good values. The counts resulted negative to total coliforms and faecal coliforms. 403 strains were analyzed; the system could identify 172 strains in 32 genera in only 50 species. The rest of strains were not found in Sherlock data base (version 6.0). Pseudoalteromonas was the genus that was more frequently isolated. The summer and autumn seasons presented more quantity of biodiversity genera. We found genera, which are mentioned as hydrocarbon degrading genera in the literature.

Keywords : marine bacteria; fatty acids; Patagonia.

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