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Acta Biológica Colombiana

Print version ISSN 0120-548X

Abstract

HERNANDEZ ESCOBAR, CARLOS; OLIVERA ANGEL, MARTHA; OSTOS ALFONSO, HENRY  and  GUERRA, MARIA TERESA. GENETIC VARIABILITY OF THE ZEBU CATTLE BREED (Bos indicus) IN THE DEPARTAMENT OF HUILA, COLOMBIA USING MICROSATELLITE MOLECULAR MARKERS. Acta biol.Colomb. [online]. 2009, vol.14, n.3, pp.173-180. ISSN 0120-548X.

The polymorphism of 11 microsatellites from zebu cattle (Bos indicus) was studied using a commercial multiplex system to estimate genetic variability. Allele frequencies polymorphism information content and heterozygosis were calculated. Allele frequencies revealed that in the analyzed sample the markers were not equally polymorphic. The average allele was 14.2 with the highest values for the TGLA122 microsatellites. The mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. This multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs and paternity test.

Keywords : alleles; microsatellite; polymorphism; zebu.

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