Acta Biológica Colombiana
versão impressa ISSN 0120-548X
MEDINA, CLAUDIA M; CRISTANCHO, DIANA e URIBE, DANIEL. Physiological Responses And Antagonistic Capacity Of Yeast Phyllospheric Isolates Obtained In Blackberry Crops (Rubus glaucus). Acta biol.Colomb. [online]. 2009, vol.14, n.3, pp. 181-198. ISSN 0120-548X.
The phyllosphere is a complex ecosystem in which despite of having a great number of associated microorganisms, few studies have been designed to understand the ecophysiological characteristics of the microorganisms inhabitant. Yeasts, as an important element of this habitat, are exposed to a variable environment, affected by the host plant, age and leaf position, availability and nutrient quality, temperature, pH, radiation and water activity. All these factors produce an important selection pressure for the establishment of natural or introduced populations of yeast which may displace other phytopathogenic fungi. In this study 80 isolates of phyllospheric yeast were obtained from two blackberry crops (Rubus glaucus). Its potential niche was determined in terms of its capacity to growth at different conditions of temperature, pH, osmotic stress and UV radiation. The osmotic stress was the most restrictive evaluated condition for the obtained yeast. Only six isolates presented a growth higher to 0.3 OD units at 405 nm, when grown at 50% or 60% of glucose. This study identified the ecological niche of ten yeast phyllospheric isolates, selected for their capacity to growth at a wide range of conditions. Four of these isolates LvF 34, LvF 43, LvF 44 and LvF 50 were selected for their antagonistic capacity against the phytopathogenic fungi Botritys cinérea. Its taxonomic determination allowed us to report for the first time isolates of Candida kunwinensis and Rhodotorula colostri with biocontrol capacity.
Palavras-chave : Yeast; phyllosphere; Rubus glaucus; ecophysiology; osmotic stress.