Acta Biológica Colombiana
versión impresa ISSN 0120-548X
Two quantitative protease assays have been developed based on the classic photographic film test. A discontinuous assay can be performed by measuring the amount of pigment remaining in the film with any image editor software. A continuous assay can be implemented by the measuring the release of silver halide salts bonded in the film using a recording spectrophometer equipped with a Peltier Cell.
Palabras llave : Protease; Enzyme; Activity assay.