Acta Biológica Colombiana
Print version ISSN 0120-548X
The polymorphism of three microsatellite systems (BMS527, BMS4440 y BMS 2113) was evaluated in 5 Colombian creole breeds: ROM (romosinuano), BON (Blanco orejinegro), CAS (casanareño), SM (San Martinero), CCC (Costeño con cuernos) and two foreign breeds: Cebu and Holstein . Thirty eight alleles in 105 studied individuals were fouund and unique alleles are reported for BON, ROM, CCC and CAS. Total expected heterocigocity which includes the two foreign breeds was 0.7228, where as observed heterocigocity varied between 0.3511 and 0.7787. Hardy/Weinberg desequilibrium was evident in some breeds in two microsatellite systems, as a result, probably of selection effects in creole and foreign breeds. The analysis of several genetic distances showed that the most distant breed was Cebu compared to the Colombian ones and to Holstein , and a cluster that shows HOL-ROM-CAS together. Mixture analysis suggests that Colombian creole breeds have a large genetic contribution from Holstein (between 8 and 22%). Finally we suggest that system BMS2113, could be useful for paternity tests in Colombian creole breeds.
Keywords : Microsatellites; Creole breeds; polymorphism.