Abstract

PEREZ C, ALEXANDER; ROJAS S, JOHANNA  and  FUENTES C, JUSTO. Endophytes Diversity of Bacteria Associated with Rootsof Colosuana (Bothriochloa pertusa) Pasture in Three Locationsof Sucre Department, Colombia. Acta biol.Colomb. [online]. 2010, vol.15, n.2, pp.219-228. ISSN 0120-548X.

The objective of this study was to isolate endophytes diversity of culturable bacteria associated with grass roots colosuana Bothriochloa pertusa (L) A. Camus in three localities of the department of Sucre, Colombia. Endophytes bacterial diversity was performed by isolation of colonies on media culture. The population density was estimated by direct counting of colonies on plate and cultural characteristics of each morphotype were obtained by observation of each colony made. We determined the correlation diversity, population density and locations, using ANOVA and principal component analysis or simple correspondence, using the statistical program R, 2009 (4.5). 20 farms livestock were sampled by locality, it was observed the presence of various bacterial morphotypes endophytes.We found significant differences between diversity (morphotypes), population density (UFC.raíz^-1) and locations. The diversity of bacterial endophytes represents only a small fraction of the total diversity present in nature; being little information we have of the presence of these microorganisms in specific agroecosystems, which is why this work becomes the first evidence of association between bacteria and roots of grass endophytes colosuana in Colombia.

Keywords : Bacteria; endophytes; roots; pasture.

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