Acta Biológica Colombiana
Print version ISSN 0120-548X
AYALA VASQUEZ, MARILUZ et al. Serological and Molecular Characterization of Potyvirus Associated to the Viral Disease of Tamarillo in Antioquia (Colombia). Acta biol.Colomb. [online]. 2010, vol.15, n.3, pp. 145-164. ISSN 0120-548X.
Tomato tree (Solanum betaceum) is an andean fruit which, due to its high nutricious value and unique organoleptic properties, has very good potential for industrial processing and international marketing. In Antioquia, Colombia, this crop faces several phytosanitary problems, of which viral infections by species of the Potyvirus genus are the most limiting. In this work, potyvirus incidence levels was evaluated in seven regions of the Antioquia province using the ELISA assay. The taxomical identity of two potyvirus associated with this crop was also determined. Serological tests demonstrate the overall presence of potyvirus in six regions, with incidence levels above 80%. The only exception was the municipality of Jardin with an incidence of only 8%. Serological test specific to PVY virus, revealed its presence in many of the infected plants. DNA sequencing of RTPCR amplicons also showed the presence of a new potyvirus for which we propose the name Tamarillo Leaf Malformation Virus (TaLMV). However, to corroborate this taxonomical hypothesis it is necessary to complete the genome sequence of TaLMV. This research represents an important advance in the knowledge of viruses infecting tomato tree. However, future investigations are required to deepen further into the individual effects of each virus as well as the transmission mechanisms and interactions with different varieties of tomato tree.
Keywords : ELISA; Potyvirus; PVY; RT-PCR; Tamarillo.