Abstract

CUELLAR GIL, JORGE ANDRÉS; CORONADO RIOS, SANDRA MILENA  and  ARRUBLA QCO, ROBERTO CARLOS. Differentiation of Mycobacterial Species by FT-IR (Fourier Transform Infrared Spectroscopy). Acta biol.Colomb. [online]. 2011, vol.16, n.2, pp.109-120. ISSN 0120-548X.

Spectroscopy Fourier Transform infrared (FT-IR) was used to differentiate 10 species of mycobacteria. Mycobacterium intracelullare and M. fortuitum (ATCC). M. flavensces, M. smegmatis, M. chelone, M. gordonae, M. triviale, M. vaccae, M. terrae and M. nonchromogenicum (IP). For gender differentiation Staphylococcus aureus, Streptococcus viridans and Streptococcus pyogenes, Klebsiella pneumoniae y Escherichia coli were incluided as controls, each species was run for triplicate in KBr and ATR. The spectra were analyzed with the method of principal components to make the first derivatives of first order (D1) in the transmission mode using KBr pellet and ATR base, and a spectral library of the first derivative of ATR was kept. The detection sensitivity was 100% with KBr and the level of differentiation was 100% in three of the four samples problems.

Keywords : mycobacteria; spectroscopy; infrared; gram negative; gram positive.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )