Abstract

BERMUDEZ SANTANA, CLARA ISABEL. Finding for a Neadle in a Haystack: Trips of Small RNAs from in silico to in vitro. Acta biol.Colomb. [online]. 2011, vol.16, n.3, pp.103-114. ISSN 0120-548X.

What is commonly known about transcriptome studies encompass the characterization and quantification of the complete set of RNA transcripts produced by a cell. In its early days these analysis mainly focused on examination of messenger RNAs or coding RNAs. More recently, due to advances in next-generation sequencing technologies, transcriptome analysis has expanded to non-conding RNAs (ncRNAs). These molecules exhibit high variety of functions in cell regulation. Through characterization of complete sets of ncRNAs is not attainable with current approaches. At present de novo identification of ncRNAs and ncRNA-derived small RNAs is a major issue in genetic analysis. One of the most important recent advances of transcriptome analysis focuses, for example, on RNA interference (RNAi) biology and its clinical application. High-performance computing, storage capability and computational modeling have been continuously developed during last years to process and model large amounts of products of next generation sequencing methods. As consequence this review describes transcriptome sequencing analysis from a historical perspective to its link to computational approaches to model ncRNAs from small RNA library data.

Keywords : Transcriptome; small RNAs; next-generation sequencing technology.

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