Abstract

ROJAS, MARCIA M et al. Antisera Production to Detect Indoleacetic Acid in Cultures of Plant-Growth Promoting Bacteria. Acta biol.Colomb. [online]. 2012, vol.17, n.2, pp.271-280. ISSN 0120-548X.

Rabbit polyclonal antisera against indoleacetic acid (IAA) bound to nitrocellulose membrane were obtained, which exhibited a high titer and specificity. The dot immunobinding technique with colloidal gold was used to detect auxin production by several strains belonging to Gluconacetobacter, Herbaspirillum, Azospirillum, Pseudomonas, Burkholderia and Bacillus genera, using culture supernatants as antigens. Moreover, auxin production was quantified by the Salkowski's method to corroborate the previous results. It was found that that all the studied microorganisms produce IAA and the feasibility of using these antisera to detect the metabolite was confirmed. Taking into account the potentialities of plant growth promoting bacteria as biofertilizers, the use of these antisera for a rapid and easy detection of IAA in bacteria associated with important crops is thus recommended.

Keywords : auxins; antiserum; dot-immunobinding; PGPB.

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