Resumo

RODRIGUEZ CUBILLOS, ANDRÉS EDUARDO; PERLAZA JIMENEZ, LAURA  e  BERNAL GIRALDO, ADRIANA JIMENA. RNA-Seq Data Analysis in Prokaryotes. Acta biol.Colomb. [online]. 2014, vol.19, n.2, pp.131-142. ISSN 0120-548X.  https://doi.org/10.15446/abc.v19n2.41010.

RNA-Seq is nowadays the method of choice for the sequencing of transcripts and transcriptomes in the field of molecular biology and gene expression assays. Until recently, this technique has allowed for the sequencing of RNA transcripts in an unprecedented scale and depth never reached in previous years; nevertheless, the reach and validity of the conclusions generated will depend strictly on an adequate experimental design and a robust analysis of the data. Given the inherent differences between prokaryotes and eukaryotes, the RNA-Seq analysis should take into account the type of organism studied. In this review we present the main factors to take into consideration when designing a consistent analysis for this type of data in prokaryotes, from the experimental design to the in silico analysis of the generated data.

Palavras-chave : analysis; experimental design; prokaryotes; RNA-Seq.

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