SciELO - Scientific Electronic Library Online

 
vol.19 número2EVALUACIÓN POBLACIONAL Y GRADO DE AMENAZA DE Chalybea macrocarpa(MELASTOMATACEAE) ESPECIE ENDÉMICA DE COLOMBIARESPUESTAS DEL ZOOPLANCTON EN UN SISTEMA TROPICAL (EMBALSE LA CHAPA, COLOMBIA) CON ALTA TENSIÓN AMBIENTAL índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Acta Biológica Colombiana

versión impresa ISSN 0120-548X

Resumen

ATENCIO-GARCIA, VÍCTOR J. et al. Evaluation of Ethylene Glycol as a Cryoprotectant in the Sperm Cryopreservation of Trans-Andean Shovelnose Catfish (Sorubim cuspicaudus, Pimelodidae). Acta biol.Colomb. [online]. 2014, vol.19, n.2, pp.271-280. ISSN 0120-548X.  https://doi.org/10.15446/abc.v19n2.41288.

The catfish Sorubim cuspicaudus cryopreservation semen was evaluated using three levels (5, 10, 15 %) of ethylene glycol (ETG). Males (n = 13) undergoing spermiation and in final maturation females (n = 6) were induced with 0.4 ml Ovaprim®/Kg, after 12 and 14 post-induction the semen was collected in 2 ml Eppendorf vials. The different cryoprotectants solutions were prepared with glucose 6 % (w/v) skimmed milk powder 5 % (w/v) and distilled water. The semen was diluted in ratio 1:3 (semen:extender), packed in macrotubes of 2.5 ml and frozen in liquid nitrogen (NL) vapor for 30 minutes, then the macrotubes were stored in cryogenic tanks submerged directly in NL (-196°C). The sperm were thawed in serological bath to 35°C for 90 seconds. The total motility, total progressivity and velocities in fresh and thawed semen were analyzed with the Sperm Class Analyzer software SCA® (Microptic SL, Spain). Fertility and hatching rates were assessed with 1.0-1.5 g of oocytes in experimental up flow incubators two liters, a completely randomized design was used. The hatching rate of fresh semen was 51,8°21 %, with no significant differences with semen cryopreserved with ETG 5 % (38.6 ° 13.9 %) (p> 0,05), while ETG 15 % (9.6 ° 2.9 %), recorded the lower hatching rate (p < 0.05). The results suggest that the cryoprotectant solution composed of ETG 5 %, glucose 6 % and powdered milk 5 % is a viable alternative for semen cryopreservation of the catfish Sorubim cuspicaudus.

Palabras clave : Colombia; cryoprotective agents; fertility; reproduction; semen analysis.

        · resumen en Español     · texto en Español     · Español ( pdf )