Resumo

JAZAYERI, Seyed Mehdi; MELGAREJO MUNOZ, Luz Marina  e  ROMERO, Hernán Mauricio. RNA-SEQ: A GLANCE AT TECHNOLOGIES AND METHODOLOGIES. Acta biol.Colomb. [online]. 2015, vol.20, n.2, pp.23-35. ISSN 0120-548X.  https://doi.org/10.15446/abc.v20n2.43639.

RNA Sequencing (RNA-Seq) is a newly born tool that has revolutionized the post-genomic era. The data produced by RNA-Seq, sequencing technologies and use of bioinformatics are exploding rapidly. In recent years, RNA-Seq has been the method of choice for profiling dynamic transcriptome taking advantage of high throughput sequencing technologies. RNA-Seq studies have shown the transcriptome magnitude, notion and complexity. From 2008, as its introduction year, the relevant reports on RNA-Seq have been multiplied by more than 2822 times just in 6 years. RNA-Seq also contributes a more accurate gene expression and transcript isoform estimation than other methods. Furthermore, some of the potential applications for RNA-Seq cannot be conducted by other methods and as yet are unique to RNA-Seq. As RNA-Seq approaches increase in speed and decrease in cost, more distinct researches are applied and become more common and accurate. RNA-Seq is a cross and interdisciplinary method that interconnects biology to other scientific topics. This article describes RNA-Seq approach, technologies, methodologies, implementation, and methods done so far in characterizing and profiling transcriptomes.

Palavras-chave : HTS High Throughput Sequencing; NGS Next Generation Sequencing; RNA-Seq, transcriptome; gene expression.

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