Abstract

ARTIGAS R, Maria D  and  FERNANDEZ DA SILVA, Rafael. Establishment of Regeneration System for Somatic Embryogenesis of Azadirachta Indica A. Juss.. Acta biol.Colomb. [online]. 2015, vol.20, n.2, pp.73-83. ISSN 0120-548X.  https://doi.org/10.15446/abc.v20n2.44200.

Azadirachta indica, is a plant with multiple forest and pharmacological application. Therefore, the establishment of in vitro culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962) supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L^-1) with 96 % and TDZ + ABA (0.02 + 1 mg.L^-1) with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term.

Keywords : 6-Benzyl-aminopurine (BAP); 2,4-dichlorophenoxyacetic acid (2,4-D); Neem; regeneration; somatic embryogenesis.

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