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Acta Biológica Colombiana
Print version ISSN 0120-548X
Abstract
GUERRERO ZUNIGA, Leonor Angélica and RODRIGUEZ DORANTES, Angélica. Assessment of (Fouquieria splendens ssp. breviflora) Cell Cultures Response Under Water Stress. Acta biol.Colomb. [online]. 2017, vol.22, n.2, pp.165-174. ISSN 0120-548X. https://doi.org/10.15446/abc.v22n2.56809.
ABSTRACT Plant cell cultures are homogenous experimental systems, highly controllable that allow the study of short and large water stress adaptations without the interference of the different tissues and development ofplants. An approach to understand these adaptations is through the presence of induced proteins; as a result of changes in genetic expression. This work analyze the response of Fouquieria splendens ssp. breviflora cell cultures exposed to abscisic acid (ABA), through the electrophoretic characterization of quantity and quality of stress induced proteins. There were recorded low molecular weight polypeptides (< 35kDa), common in experiments under ABA 10mM, followed by the association with 20 and 30mM ABA conditions, with a particularly response of cell cultures without the stress agent.
Keywords : electrophoresis; in vitro cell cultures; induced proteins.