Resumo

CAMEL PAUCAR, Vladimir; GALEANO, Esteban  e  CARRER, Helaine. In silico Analysis and Gene Expression of TgNACO1 Transcription Factor Involved in Xylogenesis and Abiotic Stress in Tectona grandis.. Acta biol.Colomb. [online]. 2017, vol.22, n.3, pp.359-369. ISSN 0120-548X.  https://doi.org/10.15446/abc.v22n3.62164.

Secondary xylem is the most abundant component of plant biomass. Therefore, knowing the genes that regulate its formation would help to design strategies for wood genetic improvement. Thus, the objective of this work was to perform computational analysis of the primary and secondary structure of the TgNACO1 transcription factor (FT) of Tectona grandis, and to evaluate its evolutionary history, conserved domains and gene expression in lignified tissues of trees with 12 and 60 years old. For this, an ion-electron interaction potential (IEP) was evaluated using the information-spectrum method (IEM) using the R-Project and SFAPS library, followed by structural modeling using the MODELLER software and visualized by PyMol program. In addition, the analysis of multiple sequence alignment and phylogeny was performed using Bioedit and MrBayes software, respectively. We also evaluated the qRT-PCR levels of TgNACO1. As results, it was found that TgNACO1 maintains a twisted antiparallel 3-sheet structure, which is compacted against an a-helix in the N-terminal region, having three a-helix domains and seven folded ((-domains. Also, through the IEM, it was demonstrated that it has about five biological functions, and mutations on amino acids with higher IEP, which leads to evolutions on genetic regulation networks. Finally, the FT TgNACO1 could play an esential role in the organization and development of the parts that make up the sapwood, such as the radial cells of the cambial zone, the vessels, fibers and the growth rings.

Palavras-chave : gene expression; method of information spectrum; protein structure; secondary xylem; transcription factor.

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