Abstract

RIVERA-URBALEJO, América et al. Contributions and difficulties of soil metagenomics and its impact on agricultura. Acta biol.Colomb. [online]. 2021, vol.26, n.3, pp.449-461.  Epub Jan 27, 2022. ISSN 0120-548X.  https://doi.org/10.15446/abc.v26n3.85760.

Microorganisms are of great interest because they colonize all types of environment, however, one of the problems we face in knowing biological diversity is that not all microorganisms are cultivable. The development of new technologies such as the generation of cloning vectors coupled with the development of high performance sequencing techniques, have favored the emergence of a new tool in science called metagenomics, which allows us to study genomes of entire communities. Since all environments are different, the type of challenge that we will face when working with metagenomics is going to change depending of the type of sample, in the specific case of soils, there are several variables, such as soil contamination with heavy metals or chemical compounds that could limit metagenomic studies. However, despite the limitations that the environment presents, with the help of metagenomics, both gene discovery and the characterization of microbial communities that positively influence plant development have been achieved, which could generate a greater impact on agriculture in the future. In this article a review of several investigations that have used metagenomics, reported in the PudMed and Google Schoolar databases was carried out, with the aim of examining the benefits and limitations of the various methodologies used in the treatment of metagenomic DNA from soil and the impact of metagenomics in agriculture.

Keywords : metagenomics; soil; agriculture; inoculants; PGPR's growth promoting bacteria.

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