Abstract

HURTADO-CLOPATOSKY, Stephany; MELGAREJO, Luz Marina; CRUZ-CASTIBLANCO, Ginna Natalia  and  HOYOS-CARVAJAL, Lilliana María. BIOTECHNOLOGICAL POTENTIAL OF PURPLE PASSION FRUIT ENDOPHYTIC FUNGI (Passiflora edulis f. edulis; Passifloraceae). Acta biol.Colomb. [online]. 2023, vol.28, n.3, pp.449-459.  Epub Sep 05, 2023. ISSN 0120-548X.  https://doi.org/10.15446/abc.v28n3.98933.

There is evidence that all plants coexist with endophytes, indicating a fundamental role that is unknown. The objective was to evaluate the biotechnological potential of endophytes from Passiflora edulis f. edulis in two aspects: as a plant growth promoter and as a control for the pathogen Fusarium oxysporum. An in vitro phase was used in which the antifungal activity in a dual culture was studied, where the UNE075 and UNE004 fungi inhibited the radial growth of the pathogen by up to 67.63 % and 63.89 %, respectively. Additionally, higher inhibition percentages were seen with UNE075 (33.78 %) and UNE098 (32.32 %) because of the action of volatile organic compounds on F. oxysporum. Likewise, the production of indoleacetic acid (IAA) as a growth-promoting compound was quantified, with notable results with UNE017 (11.99 μg/mL) and UNE022 (7.59 μg/mL). The capacity of the fungi to solubilize phosphorus was determined. UNE098 generated the greatest solubilization by reducing the pH culture medium. In the in vivo phase in the greenhouse, the effect of inoculation with endophytes on the growth of P. edulis f. edulis plants was evaluated. Biomass accumulation and leaf area were determined, where UNE067 stood out because of its effect on fresh weight, total dry weight. In general, the evaluated endophytes have biotechnological potential for use in organic crop management programs and for biological control.

Keywords : antifungal agent; biological control agent; endosymbionts; microbiota; phytopathogenic fungus.

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