Abstract

MARTINEZ RAMOS, Pedro  and  MATTAR VELILLA, Salim. Aminoglycoside resistance by aph(3’)-VIa and aac(3´)-II genes in Acinetobacter baumannii isolated in Montería, Colombia. Salud, Barranquilla [online]. 2012, vol.28, n.2, pp.209-217. ISSN 0120-5552.

Objetive: lnvestigate aminoglucoside-resistant A. baumannii strain by expressing aph(3')-Vla y aac(3')-llgenes, in a clinicfrom Monteria. Materials and Methods: Between august 2005 andfebruary 2007 were collected 17 iso-lates of A. baumannii resistant to aminoglycosides at a private clinic providing tertiary care in Monteria. Antimicrobial susceptibility was determined by minimum inhibitory concentration. Were amplified and sequenced the genes aph(3')-VIa, aph(3')-Ia, aac(3)-I, aac(3)-ll y aac(6)-la. The sequence analysis was performed with the GenBank database and BLASTX search engine. DNA of all isolates were prepared in agarose blocks and digested with the restriction enzyme Apal. Results: Sixteen of 17 isolates (94,1%) resistant to amikacin were positive for the gene aph(3')-VIa and all isolates were positive for aac(3')-II, no isolates were positive for the gene aac(3')-I, aph (3')-Ia and aac(6')-I. The PFGE pulsotypes showed 8 with two clones in 11 isolates, distributed in 7 isolates pulsotypes and 4 isolates pulsotypes ll. The other six pulsotypes included isolates unrelated. Conclusion: resistance to aminoglycosides in A. baumannii is primarily coded by the gene aph(3')-VIa gene and the aac(3')-II. Molecular epidemiological analysis showed that resis-tance to amikacin is due to the large spread of the gene aph(3')-VIa in A. baumannii isolates that in many cases as in this study cause outbreaks.

Keywords : Nosocomial pathogens; Antimicrobial resistance; PFGE.

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