Abstract

HERNANDEZ ATEHORTUA, Paula C; CHAPARRO OLAYA, Jacqueline  and  MORALES DE LA PAVA, Liliana. Genetic assemblages of axenic Colombian cultures of Giardia intestinalis. Salud, Barranquilla [online]. 2016, vol.32, n.2, pp.191-200. ISSN 0120-5552.

Abstract Objective: To genotype samples of G. intestinalis isolated from patients of three Colombian regions. Materials and methods: 22 isolates of Giardia (all of them established as axenic cultures) were examined by analyzing restriction fragment length polymorphism (RFLP) of the glutamate dehydrogenase gene (gdh). Results and conclusion: The RFLP patterns showed that all of the Giardia isolates belonged to the assembly A, specifically to AI. This result was confirmed by sequencing. This study aims to contribute to the knowledge of circulating genotypes of Giardia intestinalis in Colombia.

Keywords : Giardia intestinalis; assemblage; genotyping; gdh; RFLP.

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