SciELO - Scientific Electronic Library Online

vol.13 issue3Clinical, electrocardiographic and echocardiographic characteristics of Chagas’ cardiomyopathy in Santander’s State (Colombia) population author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Revista Colombiana de Cardiología

Print version ISSN 0120-5633


SOUTHGATE, Richard; OSORIO, Cristina; BUSTAMANTE, John  and  ALZATE, Óscar. Differential heart protein expression in diabetic type Sprague-Dawley rats. Rev. Col. Cardiol. [online]. 2006, vol.13, n.3, pp.141-148. ISSN 0120-5633.

Cardiac proteins were isolated from diabetic and wild type Sprague-Dawley rats, then fractionated by two dimensional gel electrophoresis (2D-PAGE) using isoelectric focusing and molecular weight. The resulting protein spots were stained to facilitate detection. After being converted into a digital image, the proteins on the diabetic and wild type gels were matched to each other then compared to determine levels of expression. Sixty of the one hundred and eighty proteins on the gel were removed and digested to produce peptide fragments, which were analyzed by mass spectrometry to determine their amino acid sequence. This information was entered into a protein database ( to determine the identity of the corresponding proteins. The identity of proteins differentially expressed in cardiac tissue of both groups was determined: several proteins were found to be expressed at different levels than normal in the hearts of diabetic rats, including protein tyrosine phosphatase (PTP, Q60998), very low-density lipoprotein receptor (VLDL-R, P98156), glutathione peroxidase (PHGPx, O70325), serine hydroxymethyl transferase (SHMT, P50431), adenylyl cyclase-associated protein 1 (CAP1, P40124), and telethonin (TELT, O70548).

Keywords : cardiac proteins; diabetes mellitus; mouse model; proteomics.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License