Abstract

NINO-LOPEZ, Lilibeth Caridad  and  GELVES-ZAMBRANO, Germán Ricardo. Simulating gas-liquid mass transfer in a spin filter bioreactor. Rev.fac.ing.univ. Antioquia [online]. 2015, n.75, pp.163-175. ISSN 0120-6230.  https://doi.org/10.17533/udea.redin.n75a16.

Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m³ Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating flow, bubbles breakage and coalescence were simulated by using the k-e, MRF (Multiple Reference Frame) and PBM approaches, respectively. The numerical results from different operational conditions are compared with experimental data obtained from k_La measurements and good fitting data is achieved. Interested by these simulated and experimental results CFD simulations are qualified as a very promising tool not only for predicting gas-liquid hydrodynamics but also for finding design requirements that must be implemented to optimize an aerobic bioprocessing useful for plant cell culture applications which are characterized by the constrain of achieving relatively high mass transfer conditions and avoiding cellular damage due to hydrodynamic conditions.

Keywords : bioreactor; scale up; multiple reference frame (MRF); population balance model (PBM); spin filter; biorreactor; escalado; marco de referencia múltiple; método de balance poblacional; filtro rotativo.

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