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CES Medicina

Print version ISSN 0120-8705

Abstract

MONTOYA-GUZMAN, Melissa et al. TP53 R249S mutation in patients with cirrhosis and hepatocellular carcinoma in a hospital at Medellin. CES Med. [online]. 2019, vol.33, n.2, pp.100-110. ISSN 0120-8705.  https://doi.org/10.21615/cesmedicina.33.2.3.

Introduction:

The dietary exposure to aflatoxin is a risk factor of hepatocellular carcinoma, the most frequent primary liver cancer. This risk factor was identified after in vivo and in vitro evidence of the relation between exposure to aflatoxin B1 and transversion G → T at 249 codon of the TP53 gene; as well as evidence of the synergy between hepatitis B virus chronic infection.

Methods:

the frequency of the R249S mutation of the TP53 gene was determined in 30 cases of cirrhosis and/or hepatocellular carcinoma, with liver transplantation in the hepatology unit of a hospital in Medellín, Colombia. DNA was extracted from the liver explant samples; the sequence of interest was amplified, and the mutation was detected by restriction fragment length polymorphisms.

Results:

the R249S mutation was found in 1 of the 30 samples analyzed (3.33 %); and hepatitis B virus infection was detected by serological markers in 2 of the 30 cases (6.67 %). We did not find the mutation and the presence of hepatitis B virus infection markers at the same time in any of the samples.

Conclusion:

The results suggest a low dietary exposure with aflatoxin B1 in the study population. However, it is important to take into consideration the regulation of the permissible limits of aflatoxin B1 and the inclusion in the differential diagnosis of hepatocellular carcinoma, given the heterogeneity of the conditions of the population in different regions of the country.

Keywords : Hepatocellular carcinoma; Aflatoxin B1; TP53; Hepatitis B Virus.

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