Abstract

CARRILLO-MENDIGANO, Natalia; GARCIA-ROBAYO, Dabeiba Adriana  and  OTERO-MENDOZA, Liliana Margarita. Isolation and osteodifferentiation capacity of dental pulp and periodontal ligament stem cells. CES odontol. [online]. 2015, vol.28, n.2, pp.20-34. ISSN 0120-971X.

Introduction and objective: Dental tissues have mesenchymal stem cells with capacities to differentiate in several tissues. The aim of this research was to compare the ability of osteodifferentiation of stem cells isolated of dental pulp and periodontal ligament cultured with different media. Materials and methods: 24 samples of pulp tissue and 30 samples of periodontal ligament were obtained of third molars. Stem cells isolated by the explant method were subjected to 5 osteodifferentiation conditions. Osteodifferentiation was evaluated by RT-PCR and Alizarin Red. Results: The isolated cells were characterized as stem cells by flow cytometer. Collagen type I showed the highest expression in dental pulp Stem Cells while Runx2 was increased in periodontal ligament Stem Cells. Medium 5 showed the best conditions for osteodifferentiation of periodontal ligament Stem cells while Dental pulp Stem Cells showed better osteogenic profile in medium 4. The unique difference between mediums 4 and 5 was the concentration of Ascorbic Acid 50um in medium 5 and 200um in medium 4). Conclusions: The cells obtained both pulp and ligament showed features of Stem Cells. The osteogenic capacity of cells was depending of the concentrations of the substrates applied.

Keywords : Stem cells; dental pulp; periodontal ligament; osteodifferentiation.

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