Revista Colombiana de Gastroenterologia
Print version ISSN 0120-9957
ACOSTA AMADOR, Paula Nicole et al. Molecular and bioinformatic characterization of Helicobacter pylori CagA protein using gastric biopsies of Colombian patients. Rev Col Gastroenterol [online]. 2009, vol.24, n.4, pp. 353-362. ISSN 0120-9957.
Helicobacter pylori CagA protein, the cagA gen product, has been considered as a virulence factor associated with a considerable increase risk for develops severe gastric illness. The purpose of this research was to design a molecular and bioinformatics strategy that allowed the establishment of phosphorylation status of the tyrosine residue of the CagA protein. The amplification and sequencing of the variable fragment region of cagA in the positive CagA samples were used to do the bioinformatics analysis in order to establish the characteristics of the EPIYA motifs. The presence of the EPIYA-A and EPIYA-B motifs, followed by one or two EPIYA-C repetitions, similar to those reported previously for occidental countries were set up. From the different bioinformatics applications that were employed only one group of tools proved to be useful to characterize the repeated units presents in the CagA protein.
Keywords : Helicobacter pylori; CagA protein; sequencing; bioinformatics strategy; phosphorylation motifs.