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Revista Colombiana de Gastroenterologia

Print version ISSN 0120-9957

Abstract

MARTINEZ, Julián David et al. The SD BIOLINE Rapid Test for Detection of Antibodies to HCV among High-Risk Patients. Rev Col Gastroenterol [online]. 2015, vol.30, n.3, pp.273-278. ISSN 0120-9957.

Introduction: Hepatitis C virus (HCV) infections are a public health problem throughout the world: the World Health Organization (WHO) estimates that over 180 million people are currently infected. The aim of this study is to determine the prevalence of anti-HCV in patients at high risk of infection through using a rapid test with capillary blood and confirmation of infection by PCR testing in real time. Method: Patients were enrolled in the study from among those treated in the gastroenterology unit of the Hospital Universitario de La Samaritana. This is one of the principal referral centers in the department of Cundinamarca, but it cares for patients from across the country. Hepatitis C risk factors identified included previous history of hepatitis C, transfusions, hemodialysis, major surgery (SNC, thorax, abdomen, orthopedic), drug addiction, tattooing, piercing, acupuncture, time in prison, experience as a sex worker, HIV/AIDS, sexually transmitted diseases, health care work, chronic renal failure, and time spent living in the chronic renal failure the Orinoco and/or Amazon regions. Patients referred because of abnormal liver profiles, fatty liver disease, liver masses, cirrhosis (except when due to Hepatitis C virus), ascites, and esophageal-gastric varices were also included in the study. The SD BIOLINE HCV One Step Hepatitis C Virus Test (Standard Diagnostics, Inc. Korea), which is a commercially available kit, was used. This test contains a membrane which is pre-coated with recombinant HCV antigens (core, NS3, NS4, and NS5). A Protein A colloid is combined with the serum sample which then moves along the chromatographic membrane forming a visible line showing the antigen-antibody- Protein A reaction. This test has a high degree of specificity and sensitivity. Results: Between January and October 2014, 391 patients were included in the study. Of this number, 161 were women (41%) and 230 were men (59%). Average patient age was 46.6 years, and the age range was 14 to 86 years. Four patients, three women and one man, tested positive for hepatitis C. All four diagnoses were confirmed by real time-PCR. The prevalence of HCV was 1.02%. Conclusions: This study of a population selected especially for histories of risk factors associated with HCV showed this rapid test identified HCV in more than 1% of the population. This is what the true prevalence of HCV in this population would be expected to be based on the findings of 2012 and also based on the sensitivity of the rapid test

Keywords : Hepatitis C; prevalence; rapid test.

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