Abstract

GALEANO, Carlos Hernando. Standardising Amplified Fragment-Length Polymorphisms (AFLP) for Dura oil palm (Elaeis guineensis Jacq.) and preliminary molecular characterization studies. Agron. colomb. [online]. 2005, vol.23, n.1, pp.42-49. ISSN 0120-9965.

Oil palm (Elaeis guinnensis Jacq.) plays an important role in some tropical countries’ economies; Colombia occupies first place in production in Latin-America and fifth throughout the world. The aim of palm breeding research is producing palm having high oil production, adapted to different climatic condition. Cenipalma is currently using molecular tools which can reduce environmental effect and selection time. This research was aimed at standardising the Amplified Fragment-Length Polymorphisms (AFLP) technique for Dura material from Cenipalma’s breeding programme. Modifications were made to the commercial AFLP kit protocol such as increasing initial DNA concentration to 270 ng· µL^-1. Two amplification product dilutions (1:10 and 1.50) were evaluated, 1:10 dilution being the best for the AFLP technique. It was also found that deionised distilled water was needed for staining polyacrylamide gels with silver nitrate (Milli Q®). A preliminary analysis of genetic diversity was carried out on 12 palms (Dura, Ténera and E. oleifera). Clustering and principal coordinate analysis results revealed that most Duras clustered according to where they are collected and that the American palm clustered alone when compared to African palms

Keywords : Molecular marker; genetic diversity; genetic similarity; DNA.

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