Abstract

SANCHEZ, Edgar Alexander  and  MOSQUERA, Teresa. Establishing a methodology for inducing the regeneration of pea (Pisum sativum L.) explants, ‘Santa Isabel’ variety. Agron. colomb. [online]. 2006, vol.24, n.1, pp.17-27. ISSN 0120-9965.

The effect of different 2,4-D, BAP and 2- ip concentrations on inducing the regeneration of peas from different types of explants was evaluated for use in genetic transformation as part of a project aimed at introducing resistance to Fusarium oxysporum f. sp. pisi which causes vascular wilt disease. Several types of explants were tested: cotyledons in abaxial and adaxial position, hypocotyls, epicotyls and zygotic embryos obtained from dry seeds and young seeds. These explants were evaluated in interaction with growth regulators in two types of light/cultivation conditions, the first being a 16-hour light and 8- hour darkness photoperiod and the second consisting of total darkness. Three growth regulators were tested (2,4-D, BAP and 2-ip) in three combinations and different concentrations. Zygotic embryo regeneration was obtained two light conditions, especially in the photoperiod and when applying BAP at 1 and 2.5 mg· L-¹ concentrations.

Keywords : direct regeneration; hypocotyl; epicotyl; cotyledon.

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