SciELO - Scientific Electronic Library Online

vol.24 issue1Agronomic characteristics and grain quality of the common 'popping' bean (Phaseolus vulgaris L.)Physiological implications of osmoregulation in plants author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Agronomía Colombiana

Print version ISSN 0120-9965


SANCHEZ, Edgar Alexander  and  MOSQUERA, Teresa. Establishing a methodology for inducing the regeneration of pea (Pisum sativum L.) explants, ‘Santa Isabel’ variety. Agron. colomb. [online]. 2006, vol.24, n.1, pp.17-27. ISSN 0120-9965.

The effect of different 2,4-D, BAP and 2- ip concentrations on inducing the regeneration of peas from different types of explants was evaluated for use in genetic transformation as part of a project aimed at introducing resistance to Fusarium oxysporum f. sp. pisi which causes vascular wilt disease. Several types of explants were tested: cotyledons in abaxial and adaxial position, hypocotyls, epicotyls and zygotic embryos obtained from dry seeds and young seeds. These explants were evaluated in interaction with growth regulators in two types of light/cultivation conditions, the first being a 16-hour light and 8- hour darkness photoperiod and the second consisting of total darkness. Three growth regulators were tested (2,4-D, BAP and 2-ip) in three combinations and different concentrations. Zygotic embryo regeneration was obtained two light conditions, especially in the photoperiod and when applying BAP at 1 and 2.5 mg· L-1 concentrations.

Keywords : direct regeneration; hypocotyl; epicotyl; cotyledon.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License