Abstract

DIAZGRANADOS, Cristina  and  CHAPARRO, Alejandro. Development of a regeneration system in Papa criolla (Solanum phureja Juz et Buck) var. Yema de huevo clone 1. Agron. colomb. [online]. 2007, vol.25, n.1, pp.7-15. ISSN 0120-9965.

A 46% of regeneration was obtained for explants without co-cultive using the CIP-MS medium (2 mg· L^-1 ZR, 0,02 mg· L-1 ANA, and 0,02 mg· L^-1 AG₃) developed in the International Potato Center. In order to improve the regeneration percentages, it was modified the proportion cytokinins:auxins of the CIP-MS medium, obtaining 55% callus formation having a concentration of 2 mg· L^-1 ZR and 0.04 mg· L^-1 ANA. Afterwards the effect of gibberellic acid was evaluated in this medium with 3 concentrations (0.02, 0.05, and 0.1 mg· L^-1) obtaining 87.6% callus formation and 48.6% regeneration using the medium 2 mg· L^-1 ZR, 0,04 mg· L^-1 ANA, 0.02 mg· L^-1 AG3 in explants under co-cultive conditions. This results were corroborated in a second regeneration assay under the co-cultive conditions with Agrobacterium tumefaciens LBA4404 transformed by the binary vector pNOV022: similar callus formation and regeneration percentages where obtained. It has been reported an average 4 ± 3.3 regenerants/ callus using S. phureja stems, whereas, in the present research, an average of 15.2 ± 3 regenerants/callus was reported.

Keywords : auxins; cytokinins; callus formation; tissue culture.

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