versão impressa ISSN 0120-9965
LOPEZ, Alfredo e CHAPARRO, Alejandro. A system for transformation potato plants (Solanum tuberosum sp. andigena var. Pastusa suprema) mediated through Agrobacterium tumefaciens. Agron. colomb. [online]. 2007, vol.25, n.1, pp. 16-25. ISSN 0120-9965.
It has been demonstrated that Agrobacterium tumefaciens mediated-transformation of potato (Solanum tuberosum) was depended upon the genotype. In addition, most of the proposed protocols were inefficient to transform the andigena subspecies. In this proposal, the initial processes of genetic improvement of the new Colombian variety Pastusa suprema, which highly andro-sterile characteristic is of great importance for genetically modified organisms, were handled. This variety was produced across the inter-specific hybridization of three different species of potato (Solanum stoloniferum, Solanum phureja var. Yema de huevo y Solanum tuberosum sp. andigena var. Parda pastusa). Stem explants was transformed using the vector pCambia 2301, which has the gene reporter of Β-glucoronidase and the gene of resistance to kanamycin. Percentage initial transformation was expressed as formation of callus on a selection medium of 31 ± 2.5% with a final 30% frequency using GUS assay. It is the first report of a transformation process of an inter-specific hybrid derived from three different species.
Palavras-chave : inter-specific hybrid; transformation; genotype; kanamycin; GUS.