versão impressa ISSN 0120-9965
CARDOZO, Carolina; RODRIGUEZ, Paola e MARIN, Mauricio. Molecular characterization of the Ralstonia solanacearum species complex in the banana growing region of Uraba. Agron. colomb. [online]. 2009, vol.27, n.2, pp. 203-210. ISSN 0120-9965.
Moko disease, caused by Ralstonia solanacearum, is one of the most important phytopathological problems of the banana agribusiness in Uraba (Colombia). This bacterium is featured by having high genetic plasticity, which difficults the design of control strategies. The aim of the present research was to establish the components of the R. solanacearum species complex, through the study of the genotypic features of a series of strains isolated from banana plants, weeds and soils from different plantations in Uraba. The analysis was carried out through multiple PCR and sequencing of three genomic regions (egl and fliC genes, and region 16S of 16S rDNA). The results indicate that all the studied isolates are associated to phylotype II, and most of them belong to sequevar 4. However, in the case of some strains, it was not possible to associate the PCR results with the sequevars that have been reported for race 2. Interestingly, two isolates from the department of Magdalena used as comparative controls for the bacterial population of Uraba, were found to belong to sequevar 6. These results raise important epidemiological aspects of the management of moko disease in the banana growing region of Uraba.
Palavras-chave : rDNA 16S; endoglucanase; phylotype; flageline; Moko disease; sequevar.