SciELO - Scientific Electronic Library Online

 
vol.27 número3Theories on cultivated potato taxonomy (Solanum L. sect. Petota Dumort.). A reviewClassification of 85 pea accessions (Pisum sativum L.), according to their agronomic behavior and morphological characters índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Agronomía Colombiana

versão impressa ISSN 0120-9965

Resumo

TOFINO, Adriana et al. Validation of TILLING in the evaluation of inbred progenies of irradiated cassava (Manihot esculenta Crantz). Agron. colomb. [online]. 2009, vol.27, n.3, pp.313-321. ISSN 0120-9965.

In the context set by the continuously growing agroindustrialization of cassava, and given that starch traits define tuber industrial potential, molecular tools that allow identifying variants of interest should be integrated into breeding programs for efficient selection of parental materials. The present research study compared the TILLING reverse genetics technique (modified by directly visualizing cleaved products in agarose gel) to the traditional acrylamide gel and Li-Cor platform method. Both techniques were applied to polymorphism evaluation in 10 genes with major control of the starch metabolic route. A group of 150 M2 inbred lines derived from irradiated germplasm were phenotypically identified as probable mutants and analyzed by TILLING. Only 30% of the primers used produced good quality data, for most of them amplified more than one target. Additionally, visual detection of SNPs in agarose was not achieved in mixtures of DNA templates nor in individual samples, so the results cannot be said to correspond with those obtained by Li-Cor genotyping.

Palavras-chave : primer synthesis; Single Nucleotide Polymorphism (SNP); reverse genetics; induced mutations; starch quality; genotyping platform.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons