Resumo

RACHE C., Leidy  e  PACHECO M., José. Micropropagation of Espeletiopsis muiska (Cuatrecasas), 'frailejon' from Rancheria Natural Park-Boyaca, Colombia. Agron. colomb. [online]. 2009, vol.27, n.3, pp.349-358. ISSN 0120-9965.

The study developed a protocol for the micropropagation of Espeletiopsis muiska, an endangered species of ecological, medicinal and industrial importance. Sexual embryos were cultured in vitro in Murashige and Skoog (1962) complete and incomplete (reducing macrosalts by half) mediums, with and without growth regulators. Initial proliferation was carried out by culturing apical stem segments in medium with individual and combined addition of auxins and cytokinins; also evaluating the effect of different concentrations of hydrolyzed casein and sucrose. Rooting was promoted by growing the shoots in culture medium with 3-indole-butyric acid. Plantlet acclimatization, which started in incubation room and finished in greenhouse, made use of different substrates. The highest percentage of germinated embryos (91%) was obtained in growth regulator free medium. The largest number of normal appearance shoots per explant (5.96) was observed in proliferation medium containing 2.46 μM 3-indole-butyric acid plus 0.044 μM 6-benzylaminopurine. During the root taking phase, the medium containing 0.49 μM 3-indole-butyric acid promoted a rooting percentage of 54.1%. After 30 days of acclimatization, 60% of the plantlets transferred to soil enriched with organic matter resulted viable.

Palavras-chave : cytokinins; auxins; sucrose; hydrolyzed casein; propagation.

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