SciELO - Scientific Electronic Library Online

 
vol.29 issue3Environmental effect of conventional and GM crops of cotton (Gossipium hirsitum L.) and corn (Zea mays L.)Identification in silico of SSR markers for genotyping Hevea sp. clone gardens in Colombia author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Agronomía Colombiana

Print version ISSN 0120-9965

Abstract

VEGA-VELA, Nelson Enrique  and  CHACON SANCHEZ, María Isabel. Isolation of high-quality DNA in 16 aromatic and medicinal Colombian species using silica-based extraction columns. Agron. colomb. [online]. 2011, vol.29, n.3, pp.349-357. ISSN 0120-9965.

Aromatic and medicinal plant species are a valuable resource for research and development of pharmaceutical, cosmetic, crop protection and nutritional agents, due to the high amount of bioactive phytochemicals that they contain. However, these compounds are a major obstacle in the isolation of high-quality DNA suitable for genetic analyses. In this paper, we report a protocol that optimizes the use of the cationic detergent CTAB and the reductant β-mercaptoethanol in cell lysis. The elimination of plant secondary metabolites such as polysaccharides and polyphenols, that typically co-isolate with DNA, was achieved using the chemical denaturing properties of the guanidinium cation, which together with the adsorbent chemical specificity of the silica, resulted in the purification of high-quality DNA suitable for digestion with restriction enzymes and optimal for PCR amplification of AFLP-type molecular markers. This protocol was evaluated on 16 Colombian aromatic and medicinal plant species promising for their essential oils. The results allow suggesting that this procedure might be appropriate for other species, tissues and sample types recalcitrant to DNA extraction

Keywords : chaotropic agent; guanidinium cation; silica-based extraction columns; nucleic acid extraction kit; guanidinium salts; molecular markers.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License