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Agronomía Colombiana

versão impressa ISSN 0120-9965


MORILLO C., Yacenia et al. Molecular characterization of 93 genotypes of cocoa (Theobroma cacao L.) with random amplified microsatellites RAMs. Agron. colomb. [online]. 2014, vol.32, n.3, pp.315-325. ISSN 0120-9965.

Random amplified microsatellite (RAMs) markers six were used to characterize 93 genotypes of cocoa in Tumaco (Colombia). Hundred twenty seven bands were generated. The number of polymorphic loci varied between 11 and 25 for the AG and TG primers, respectively. This study differentiated the 93 genotypes into six groups with a 0.53 similarity, 0.28 mean heterozygosity (He) for the population, and 0.12±0.02 genetic differentiation coefficient or Fst. A significant level of genetic diversity was evident in the T. cacao genotypes. This resource would benefit selection programs of individual trees or plant breeding programs. The genotypes clustered in a large proportion in accordance with the collection zone. This characteristic was associated with collection zones and along the rivers in the municipality of Tumaco. The RAM technique proved to be a useful tool for the determination of genetic diversity in Theobroma species.

Palavras-chave : Malvaceae; molecular markers; heterozygosity; clonal multiplication; genetic diversity.

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