SciELO - Scientific Electronic Library Online

 
vol.32 número3Pan tropical biomass equations for Mexico's dry forestsThe history of research on oil palm bud rot (Elaeis guineensis Jacq.) in Colombia índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Agronomía Colombiana

versão impressa ISSN 0120-9965

Resumo

ANGEL, Jorge Evelio et al. Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia. Agron. colomb. [online]. 2014, vol.32, n.3, pp.377-389. ISSN 0120-9965.  http://dx.doi.org/10.15446/agron.colomb.v32n3.44069.

Citrus huanglongbing (HLB) is the most destructive citrus disease. Two of the three known HLB-associated Candidatus Liberibacter species were recently found to be present in the Americas. In this study, eggs, nymphs and adults of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and suspect citrus plant materials were collected in 25 municipalities in the departments of Cundinamarca, Santander, Valle del Cauca, Meta and Quindio (Colombia). The detection sensitivity, specificity and assay performance of the 16S rDNA-based real-time PCR (qPCR) were validated for the field survey of the disease in Colombia. The validation confirmed the reliability and robustness of the real-time PCR method for the detection of HLB bacteria in host citrus plant tissues and the vector D. citri. The diagnosis was performed for Candidatus Liberibacter asiaticus (Ca. L. asiaticus) and for Candidatus Liberibacter americanus (Ca. L. americanus) on 168 citrus plant material samples and 239 insect samples. Neither Ca. L. asiaticus nor Ca. L. americanus were detected in the host plants or insects vector, confirming the absence of the disease in the citrus-producing areas of Colombia.

Palavras-chave : Diaphorina citri; HLB; Psyllidae; qPCR; 16S rDNA.

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )