Abstract

SAAVEDRA, Stephy et al. Optimization of lactic acid production by Lactobacillus plantarum strain Hui1 in a medium containing sugar cane molasses. Agron. colomb. [online]. 2021, vol.39, n.1, pp.98-107.  Epub Sep 14, 2021. ISSN 0120-9965.  https://doi.org/10.15446/agron.colomb.v39n1.89674.

The aim of this study was to optimize lactic acid production by a native strain (Huil) of Lactobacillus plantarum isolated from a Peruvian Amazon fruit (Genipa americana) in a medium supplemented with an agroindustrial by-product such as sugar cane molasses. Optimization was performed though one-factor-at-a-time studies followed by the Placket-Burman and central composite designs. The data were analyzed by using the Statistica® 10 software. Several carbon, nitrogen and ion sources were tested, and the optimum concentration of lactic acid achieved was 84.2 g L-1 in a medium containing as follows (in g L-1): meat extract, 18.69; tryptone, 7.88; sugar cane molasses, 140; calcium carbonate, 15; dipotassium phosphate, 1; manganese phosphate, 0.03; sodium acetate, 5, and magnesium sulphate, 0.2. In addition, a high degree of conversion from sugar cane molasses to lactic acid was obtained (Yp/e 0.898 g g-1). These results indicate the potential of Lactobacil-lus plantarum strain Hui1 to produce lactic acid in a medium supplemented with sugar cane molasses, an underutilized industrial by-product.

Keywords : fermentation; response surface methodology; byproducts; industry.

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