Abstract

CASTILLO LEON, John Jairo; NOVOA, Leidy Viviana; MARTINEZ ORTEGA, Fernando  and  ESCOBAR RIVERO, Patricia. Carbon nanotubes-chitosan in HOS and THP-1 cells. Rev. Univ. Ind. Santander. Salud [online]. 2011, vol.43, n.1, pp.21-26. ISSN 0121-0807.

Introduction: Carbon nanotubes (CNT) are nanometer-sized structures used in medicine in the treatment of diseases, mainly in drug delivery in therapies against cancer. Objectives: To Study the internalization of carbon nanotubes modified with chitosan (CNT-CH) in human osteosarcom cells (HOS) and human monocytes of acute leukemia (THP1). Materials and methods: The CNTs solubilized in chitosan 30% were characterized spectroscopically by UV-Vis, fluorescence and Raman. HOS cells and THP-1 were treated with CNT-CH, the internalization was evaluated by Giemsa staining with light microscopy, and cytotoxicity was determined using Alamar Blue assay. Results: Raman and fluorescence spectra showed the functionalization of the CNT with chitosan. After 24 h the NTC were internalized in the cell lines showing a cytoplasmic location and were not cytotoxic in any of both cell lines types. Discussion: The properties of CNT-CH on cells allowing it to be potentially used as drug carrier Salud UIS 2011; 43(1): 21-26.

Keywords : Carbon nanotubes; chitosan; cell internalization cells; macrophages.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )